FastQCFastQC Report
Fri 29 Apr 2016
ERR1042650.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042650.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733104
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT466992.6945295839141794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT425772.456690423656053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT258231.4899855980945171No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254181.4666171216499415No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132610.7651589287197998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA115410.6659150287576511No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62260.3592398378862434No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61570.35525854189938977No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54670.31544558203085327No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40240.23218456595795753No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39640.22872256944765001No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA34160.1971030013201747No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA33260.19191000655471338No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA32550.18781331068418283No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25940.1496736491289617No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22430.1294209695436627No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.12116987786076312No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20990.12111217791892466No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA19780.11413048495647117No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA17930.10345599571635633No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA17470.10080179839178723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC3050.020.6229528
CGCGATA555.144542E-420.18181814
GACGGAC3150.019.9682547
GGTATCA129900.019.8956891
GCGCAAG3200.019.656251
CGGACCA3300.019.6212129
GCGTTAT2400.018.51
TGCGCGA609.239395E-418.512
GAACAGT11900.018.0336131
GCCGATA852.7244076E-517.4117642
ACGGTGT752.0680294E-417.26666629
CGTATAC650.001580371517.0769233
GCGATAT650.001580371517.07692315
AACAGTG12550.016.952192
GTCGGGA2850.016.8771932
TTGACGG3450.016.62318833
GAGTATG4500.016.4444451
GTATGGT3950.016.3924053
AGTATGG4200.016.2976192
GCAAGAC5000.016.2799993
Produced by FastQC (version 0.11.5)