FastQCFastQC Report
Fri 29 Apr 2016
ERR1042648.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042648.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18931069
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT426750.2254230862504384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT377320.1993125691951152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT277110.146378421630601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128600.025.8510861
GTATCAA189850.017.5304182
GACGGAC21250.013.4070597
ACGGACC22650.013.150118
TCAACGC256150.013.0363075
ATCAACG257050.013.0266484
GTATTAG37900.012.9841691
TATACTG54950.012.9281175
TATCAAC267400.012.8545253
TACCGTC15350.012.7752447
ATAACGC13350.012.74906353
CGCGCTA12850.012.66926124
CAACGCA264500.012.6317586
TCTATAC29300.012.5648463
TTAGACT21700.012.5322574
TAACGCC10550.012.4502374
AACGCAG272050.012.3764017
CTTACAC34800.012.1738513
CGAACGA8850.012.12429316
GTACATG385450.012.0373591
Produced by FastQC (version 0.11.5)