FastQCFastQC Report
Fri 29 Apr 2016
ERR1042647.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042647.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17459257
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT609270.34896674010812717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT576120.3299796778293601No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT355770.20377155797637894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210930.12081270125068896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176450.025.079061
GTATCAA259400.017.1520822
GACGGAC26450.015.8071837
ACGGACC26600.015.7180448
TAACGCC15250.014.6786894
TACGACG15700.014.6114655
TATACTG42050.014.4304395
AAGACGG34750.014.3208645
CGACGGT15700.013.9044597
ACGACGG16200.013.7037046
CGAACGA11150.013.60538216
TCGTTTA14550.013.60481130
CGGACCA31600.013.5822789
ATTGACG24500.013.28979632
CGTCGTA13550.013.24354310
ATAACGC19200.013.1041663
TCTATAC23350.012.9935753
CCGATAA13450.012.9293689
TACCGTC17050.012.9120247
TAGACAG35900.012.8830095
Produced by FastQC (version 0.11.5)