FastQCFastQC Report
Fri 29 Apr 2016
ERR1042646.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042646.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16211992
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT625360.3857391491434242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT580250.3579140675618394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT357570.22055895413715967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213070.13142740262886882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171750.026.745561
GTATCAA250600.018.4409432
TATACTG34600.015.3453755
ACGGACC18950.014.5461748
GACGGAC19650.014.027997
CGAACGA8750.013.10857216
TACCGTC13350.013.02621657
TATCAAC364000.012.9195053
ATCAACG359150.012.8467214
TCAACGC359650.012.8340055
GTATTAG32400.012.7330251
AACGCAG362300.012.7094957
TCTATAC24050.012.6153853
CAACGCA366750.012.5552846
TACGACG10650.012.5070425
CTACACT47500.012.3073684
TTATACT35200.012.2457394
TACACTG66150.012.19355
GTAAACG13050.012.1915727
TAGACAG32850.012.051755
Produced by FastQC (version 0.11.5)