FastQCFastQC Report
Fri 29 Apr 2016
ERR1042645.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042645.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14616339
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT463030.31678931365781815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT429000.2935071497725935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT297160.20330672407091818No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166070.11361942275695713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137300.026.9617611
GTATCAA205300.018.1485632
TATACTG35150.014.8421075
TCTATAC20050.014.7630923
TAACGCC9950.014.6884424
TACCGTC12250.013.7428577
TACGACG11100.013.3333335
CGTCGTA10100.013.18811910
TAGACAG31450.013.1764715
CGACGGT11050.013.0588247
CGAACGA6950.012.776978516
TATCAAC297050.012.7173863
ATCAACG294900.012.67209154
CGCGCTA13650.012.60439624
TCAACGC295750.012.598145
ACCGTCG10800.012.504638
ACGACGG11550.012.4935066
TAGACTG20150.012.4863535
TCTAGCG8300.012.48192828
ACGGACC18100.012.4696138
Produced by FastQC (version 0.11.5)