FastQCFastQC Report
Fri 29 Apr 2016
ERR1042644.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042644.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13676569
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT549450.40174549625713873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT541020.3955816696424374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT336110.24575608107559724No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204410.14945999979965735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165950.025.160891
GTATCAA243550.017.1820982
TATACTG30150.015.4626865
ACGGACC18100.014.8204428
CGAACGA8650.014.54335216
TACGACG12300.014.4390245
ACGACGG12150.014.0082316
CGACGGT11950.013.9330547
GACGGAC18600.013.9247317
TCGTTTA9600.013.68229230
CGCGCTA14650.013.25938524
TAACGCC11400.012.9824564
GTATTAG23250.012.96989251
CCGATAA9850.012.9593929
ACGAACG9650.012.8445615
ACGCGCG15150.012.82178221
TACCGTC14200.012.6373237
CTATACT22950.012.5751644
CGTCGTA11600.012.43965510
GTATAAG23900.012.3849361
Produced by FastQC (version 0.11.5)