FastQCFastQC Report
Fri 29 Apr 2016
ERR1042643.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042643.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14594815
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT481690.3300418676084623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT412260.28247017862165436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT287860.19723442880228356No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164590.11277292654959999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147200.026.3675271
GTATCAA214700.018.163952
CGCGTAT2100.015.85714212
TATACTG34650.015.4834065
TACGACG11450.015.0262015
GTATTAG28950.014.8894641
ACGGACC20500.014.7097568
CGAACGA9700.014.49484516
GACGGAC19750.014.4253177
ACGAACG9950.014.13065315
CGACGGT11400.013.6315787
CGCGCTA16400.013.423779524
TCAACGC291100.013.3967715
TATACCG5800.013.3965515
TAGACAG31700.013.3643535
AACGCAG295300.013.2688797
ATCAACG294550.013.2587014
TATCAAC299300.013.1904433
ACCGTCG13750.013.1854548
TACCGTC14350.013.1498267
Produced by FastQC (version 0.11.5)