FastQCFastQC Report
Fri 29 Apr 2016
ERR1042642.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042642.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13113735
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT485940.37055804467605913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT394890.3011270244518438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT227400.17340597472802372No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163270.12450304966510305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137550.027.0338061
GTATCAA190100.019.6580732
CGAACGA8450.017.51479116
ACGGACC20350.016.4545468
GACGGAC19800.016.351017
CCGATAA9900.015.6969699
ACGAACG9850.014.83756315
TAACGCC10600.014.8349064
TATACTG30450.014.8243035
TACGACG11750.014.85
AAGACGG26200.014.6164115
CGCGCTA12850.014.25291824
ATCAACG270450.013.9887224
TATCAAC271200.013.9705023
AGACGGA24500.013.9693886
TCAACGC269850.013.9444135
ATAACGC12800.013.8753
CAAGACG29000.013.6517254
CGGACCA24200.013.6074389
CAACGCA275850.013.600876
Produced by FastQC (version 0.11.5)