FastQCFastQC Report
Fri 29 Apr 2016
ERR1042640.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042640.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21882203
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT661780.30242841637105733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT552220.25236033136151786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT436510.19948174322302012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257410.117634408199211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187250.028.868891
GTATCAA287850.018.850272
TATACTG53650.015.3448275
TAACGCC11750.014.84
ATAACGC16650.013.8888893
TACGACG13650.013.5531145
TCAACGC403300.013.4862385
ATCAACG404650.013.4732494
TATACCG8800.013.4545455
TATCAAC409050.013.4368653
CAACGCA413850.013.1379736
TCTATAC32350.012.9242663
CGCGTAT3150.012.92063412
TAGACAG47850.012.7586215
AACGCAG431450.012.6663577
ACGGACC22350.012.664438
TAGACTG26150.012.6634815
ACGCGCG23400.012.64957221
GTACATG657150.012.4966141
TCTAGAC26700.012.471913
Produced by FastQC (version 0.11.5)