FastQCFastQC Report
Fri 29 Apr 2016
ERR1042639.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042639.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23255132
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT950780.40884738904083623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT837230.3600194572105633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT612800.26351172721788896No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414680.1783176289861524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA248450.028.5262621
GTATCAA383600.018.6157462
GACGGAC29500.014.6118657
TATACTG62600.014.4512775
ACGGACC30850.014.1523518
TACGACG17950.014.1197785
ATACGCG2850.013.63157930
TCTATAC36100.013.1191143
TATCAAC562200.012.8598363
TAATACT61900.012.8513734
ATCAACG558750.012.8432214
CGAACGA15900.012.79874116
TCAACGC561700.012.7823565
AAGACGG40250.012.6397525
TAGACAG49800.012.5190765
ACGCTGA48700.012.49794711
CAACGCA576750.012.4391856
GTACATG814700.012.3257641
AACGCAG587800.012.243117
CGCGCTA32500.012.238461524
Produced by FastQC (version 0.11.5)