FastQCFastQC Report
Fri 29 Apr 2016
ERR1042638.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042638.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13354595
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1677831.256369062483737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1594061.1936415892806933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT902620.6758872133524079No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT652730.48876809817145334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA436550.024.5748481
GTATCAA631100.017.1427672
CGAACGA12700.015.58661516
ACGGACC17150.015.5335298
TATACTG25500.014.8725495
CGCGCTA26050.014.62955924
ACACGCT28550.014.4500889
ACGCGCG26600.014.39661621
CGGACCA20050.013.8403999
GCGCGCT27700.013.75812223
CTAACGC3500.013.7428573
AAGACGG23500.013.6191495
GACGGAC20650.013.5278447
TCCGATA15200.013.2664488
CGCGCGC29450.013.19185122
ACGCTGA32650.013.0888211
TAACGCC15700.012.9617834
CGATAAC15700.012.96178310
CTAGCGG7850.012.96178329
TCTATAC16700.012.9610783
Produced by FastQC (version 0.11.5)