FastQCFastQC Report
Fri 29 Apr 2016
ERR1042637.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042637.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11581736
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT951770.821785266043018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT924910.7985935787173875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT540700.4668557459779777No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366270.3162479269083667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA263300.024.8797951
GTATCAA381250.017.2844582
CGAACGA12300.016.39430816
GACGGAC18400.016.2880447
ACGGACC18550.016.2560658
TACGACG12300.014.7398385
TCTATAC15350.014.5830613
CGGACCA21600.014.3032419
CTAGCGG8200.014.21341529
TCTAGCG8350.013.95808428
TATACTG27100.013.7896685
GCGGGTA11300.013.75221323
GCGCAAG23700.013.6603371
TAACGCC12250.013.5918374
CGATAAC15000.013.56666810
CTAGTAC9150.013.5464483
CTTACAC20850.013.4868113
ACGAACG14550.013.47766315
AGACGGA23650.013.4545456
CGGGTAA11050.013.39366424
Produced by FastQC (version 0.11.5)