FastQCFastQC Report
Fri 29 Apr 2016
ERR1042636.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042636.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433168
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT738473.0350144338574236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT587762.4156161843325243No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252851.0391801963530674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT243961.0026434672821605No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA76760.3154734897056019No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA62750.2578942350055566No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60060.24683868931368486No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53940.2216862953976051No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46050.1892594346136395No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42270.17372413248900198No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41820.1718746917598785No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA33350.13706410736948701No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29370.12070683158746129No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.11002117404141432No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.1063633912660367No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA25380.10430845712256613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA705.1057905E-621.14285914
TAGTTCG705.1057905E-621.1428599
AGTTCGC902.1542273E-618.510
ACGGACC3650.018.2465748
CTAGTAC2250.018.0888883
GAGCGTA1156.413575E-817.6956526
TACCGAC1851.8189894E-1217.0000027
TACGACG1757.2759576E-1216.9142865
CGGACCA3850.016.8181829
GGTATCA224200.016.7671721
GCGGGTA3350.016.56716323
CGTATTA1352.2226232E-816.44444315
CGGGTAA3400.016.3235324
CGATCGT1251.6598278E-716.27999913
AACAGTG25100.016.1414362
TATACTG3100.016.1129025
TCTATAC1851.8189894E-1116.0000023
GACGGAC4050.015.9876557
CGAACGC957.064856E-515.57894830
AATCGAC957.064856E-515.5789486
Produced by FastQC (version 0.11.5)