FastQCFastQC Report
Fri 29 Apr 2016
ERR1042635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8259394
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1633991.978341268136621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1513201.8320956719100698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT829981.0048921264683583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT718640.8700880476218957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA229050.27732058792691083No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224220.27147270126597667No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147350.17840291914878986No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127690.15459972002788583No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT112550.1362690773681459No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112060.13567581350399316No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107230.12982792684305894No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA96850.11726041886365997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA352350.029.0823021
GTATCAA509100.020.3023952
TATACTG11500.016.7304345
ACGGACC10100.016.6683188
GTACTAG6550.016.6641221
GTAAACG9250.016.027
TGCGACG4750.015.96842322
CGAACGA10150.014.94581316
CGCGCTA22800.014.84868524
GACGGAC11400.014.7675457
ACGCGCG22750.014.55604421
CGAGCCG14550.014.49484515
CGCAAGA12050.014.4315352
CAATGCG5700.013.95614119
GCGCGCT24200.013.91322423
CGACTAT4300.013.76744236
TTACTCG3500.013.74285819
TCTATAC8250.013.6787883
CGCGCGC24900.013.67068322
TATCAAC767150.013.5310563
Produced by FastQC (version 0.11.5)