FastQCFastQC Report
Fri 29 Apr 2016
ERR1042634.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042634.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6765280
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT197820.2924047489534801No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT180500.2668034434642764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134010.19808492774874062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66450.021.4371721
GTATCAA94350.015.0588242
TATACTG10200.014.8725495
TCTAGCG5000.014.05999928
GTATTAG15050.013.8903651
CCTATAC9700.013.7319583
GTACTAG5400.013.7037031
GTAAACG5450.013.57798227
TACCGAC2051.4279067E-913.5365867
TAGACTA6050.013.4545465
TTAGACT6250.013.324
TACCGTC7300.013.1780827
TATACCG3400.013.0588245
TAATACT14600.013.051374
CGTCGTA6250.012.72810
GTAAGAC8900.012.6797763
GTGTAAG13300.012.6578941
GTATAGG10100.012.6386151
CTAATAC14700.012.5850333
ATTAGAC5600.012.5535723
Produced by FastQC (version 0.11.5)