FastQCFastQC Report
Fri 29 Apr 2016
ERR1042633.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042633.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8821088
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT304230.3448894286056323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT268970.30491703517752006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT201080.22795373994681836No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110190.1249165635803656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94650.022.1452711
GACGGAC10100.017.0346537
ACGGACC11100.015.6666678
CTAGCGG5200.015.65384629
GTATCAA134600.015.5037152
GTATTAG18350.015.1226161
TATACTG16850.014.05341155
TCTAGCG5900.013.7966128
CGGACCA12500.013.7649
TAACGCC7150.013.7132864
TACCGTC7950.013.2641517
CGCAAGA14950.012.9933112
CGCAATA6700.012.97761236
ATTGACG10900.012.55963332
ATAACGC9450.012.52913
AGACGGA14100.012.4645396
ATACCGC10550.012.45023727
TCTATAC12200.012.4344263
GTACATG197250.012.2583021
AACGCAG171500.012.2542277
Produced by FastQC (version 0.11.5)