FastQCFastQC Report
Fri 29 Apr 2016
ERR1042632.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042632.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7020964
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT207880.2960846972011251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT196780.28027490242080716No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT137190.19540051764971306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79750.018.4188081
GTACTAG4300.017.6395341
GACGGAC7750.016.4709667
TATACTG12050.015.96680455
CTAGTAC4400.015.5568183
CTAGCGG5800.015.31034529
TCTAGCG5850.015.17948728
TATACCG3700.015.05
ACGGACC9300.014.7204298
TACACCG7200.014.1319455
CCGTTCG1203.3050826E-513.8749999
CGCAATA6950.013.84172736
ATACCGC11050.013.72850727
ATAACGC7400.013.53
GTAAACG6200.013.42741927
TTTAGCG4200.013.21428526
TACCGTC6350.013.1102367
GTATCAA111250.012.9874152
TAACGCC6000.012.954
GTATTAG15900.012.9150931
Produced by FastQC (version 0.11.5)