FastQCFastQC Report
Fri 29 Apr 2016
ERR1042631.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042631.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6325303
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT181340.2866898233966025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT165290.2613155448837787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118580.1874692801277662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61250.018.9681631
ACGGACC7850.014.3757968
CTAGCGG4350.013.60919629
GTATCAA85500.013.5883042
TCTATAC6050.013.4545453
GACGGAC8150.013.1656467
GTATTAG13100.013.1335881
TATACTG12600.013.067465
TCGTTTA4400.013.03409130
TAACGCC5300.012.9150934
GTATAGA11700.012.8076931
GTACATG124150.012.7704391
TTAATAC8900.012.6797763
AAGACGG12550.012.52988155
AGACGGA10950.012.5022846
TACCCCG4300.012.4767445
TTTAGAC5800.012.4396553
TCTAGAC7000.012.4214293
TACATGG124450.012.3382882
CGCAATA5100.012.33333336
Produced by FastQC (version 0.11.5)