FastQCFastQC Report
Fri 29 Apr 2016
ERR1042624.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042624.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5940248
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94870.1597071368064094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81290.13684613841038287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66800.11245321744142668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.023.0649341
GACGGAC8650.016.682087
TAACGCC6450.015.4883714
TCGTTTA5600.015.19642830
GTATCAA52750.015.115642
CGAACGA2950.015.05084716
ACGGACC9800.014.9132668
ATAACGC7000.014.5357153
GTATAGA10250.014.4390241
TAAACGC6000.014.18333328
GTGTTAG7200.013.8751
GTATAGG7900.013.8164561
GTAAACG6150.013.53658627
ATACCGC9950.013.38693427
CGGACCA11350.013.3656399
TACGTCG1805.171023E-813.3611115
TCTATAC7700.012.9740263
CTAGACT6150.012.934964
GTATAAG9300.012.9301071
AGACGGA12050.012.8962656
Produced by FastQC (version 0.11.5)