FastQCFastQC Report
Fri 29 Apr 2016
ERR1042623.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042623.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6760373
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133150.19695658804625127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116750.17269757156890603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT90720.13419377895272938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.023.4988461
GTATCAA63950.015.7083662
TAACGCC5350.015.5607494
GTATTAG11300.015.0619461
TATACTG12400.014.7701615
ACGGACC7750.013.84516058
ATAACGC6450.013.7674423
TCGTTTA5750.013.51304330
TAGACAG13100.013.416035
TAATACT11900.013.0588234
GACGGAC7800.013.0448727
TCTACAC13600.012.6507363
GTACATG118800.012.2087541
TCTAGAC8200.012.1829273
CGCACTA3650.012.16438429
TACACTG21750.012.1632195
TATATAC13650.012.0622713
GTATAAG11200.012.0580351
CTACACT19250.012.0129874
ATCAACG82600.012.0048424
Produced by FastQC (version 0.11.5)