FastQCFastQC Report
Fri 29 Apr 2016
ERR1042622.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042622.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6846093
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT114440.16716103622898493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101180.1477923247609987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79920.11673811617808871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39350.021.5794161
CTAGTAC4950.017.5656553
GACGGAC8200.016.2439047
GTACTAG4150.015.1566251
ACGGACC8700.014.88505658
GTACGAC2500.014.7999993
TATACTG14400.014.6458335
TAGTACT7200.014.6458334
GTATCAA59500.014.14705852
GTATTAG12800.013.730471
TAACGCC6000.013.2583334
TCTAGCG4650.013.12903228
ATAACGC7300.012.9246583
CGGACCA10100.012.8217819
GTATAGA10150.012.7586211
TACGACG4650.012.7311825
CTAGCGG4650.012.73118229
GTATACG3550.012.5070431
GTACATG113950.012.4848611
TTATACT15150.012.4554464
Produced by FastQC (version 0.11.5)