FastQCFastQC Report
Fri 29 Apr 2016
ERR1042621.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042621.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5738315
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98990.17250708613939805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88150.15361652331738498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65620.11435412660336702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45250.018.6839771
GTACTAG4350.017.862071
GACGGAC6650.016.1353387
TATACTG10400.015.4759625
CTAATCG2251.8189894E-1214.826
ACGGACC7550.014.4569548
GCGTTAT5100.014.14705851
TAGACCG1051.6574495E-414.0952395
AACGGTA2151.9826984E-1013.76744218
TCTATAC7850.013.668793
TACACTG17950.013.5013935
GTAAACG4250.013.49411827
GTATCAA62250.013.492372
TATACCG2800.013.2142855
CTTACAC10150.013.1231533
CGTTATT4800.013.1041672
CTACACT16250.012.9784634
ATTGACG5550.012.66666732
GGGTACG2204.096364E-912.6136361
TTATACT12250.012.5346944
Produced by FastQC (version 0.11.5)