FastQCFastQC Report
Fri 29 Apr 2016
ERR1042620.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042620.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4175433
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.017.4326931
CGTAATA1508.11724E-814.82
CCGTTCG1152.2124901E-514.4782619
CTAGTAC3850.014.4155843
TATACTG6550.013.8396955
TACTAGG5100.013.7843132
TATACCG2151.9826984E-1013.7674425
TTATACT6150.013.5365864
CGCGCTA3450.013.40579724
ATGCGAA1951.0282747E-813.28205211
TAGGTCG1703.7381324E-713.05882421
TTATACC4700.012.9893624
GTACTAG3300.012.893941
TACTCCG4450.012.8876415
GTATCAA20450.012.8459652
TACGACG2753.6379788E-1212.7818175
TTTATAC5550.012.6666673
GTATACG2358.112693E-1012.5957451
TAGACAG6200.012.5322585
GTGTAAG7400.012.5000011
Produced by FastQC (version 0.11.5)