FastQCFastQC Report
Fri 29 Apr 2016
ERR1042619.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042619.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6700789
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT220860.32960297660469534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT199690.2980096821434013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT148360.22140676269615414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65500.021.8045791
TCTAGCG6150.015.9430928
CTAGCGG6350.015.14960729
GTATCAA95100.014.9789692
GTATTAG10800.014.9027781
CGTATAC3300.014.5757573
TCGTTTA8300.014.2650630
TATACTG10750.013.9395345
TACGACG7800.013.7564115
CGAACGA5800.013.71551716
GTAAACG8750.013.53142827
GTACTAG4800.013.4895831
ACGGACC11800.013.3262718
CGAGCCG12500.013.17215
TAACGCC9600.013.1041664
CGCAATA7700.012.97402636
TAGTACT9100.012.8076924
TACCGCA12650.012.7233228
GTATACT5100.012.6960784
GACGGAC12450.012.6305227
Produced by FastQC (version 0.11.5)