FastQCFastQC Report
Fri 29 Apr 2016
ERR1042618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712460
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72870.19628494313743447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT65380.17610964158536388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42830.11536824639188031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30050.021.1164741
TCTAACG1601.8189894E-1218.52
CTAACGC1653.6379788E-1217.9393943
CTAGTAC3350.017.6716423
TAGGTCG2750.016.81818221
TATACCG1901.8189894E-1216.5526315
GACGGAC3950.016.3924057
GTACTAG2800.015.8571421
GTATCAA40500.015.5308652
TCTAGCG3000.015.41666728
TAACGCC4350.015.3103444
CGCAATA2950.015.05084736
TAGTACT4800.015.0312494
TGCGACG1904.5656634E-1014.60526422
CTAGCGG3050.014.55737629
ATTAGAC3250.014.2307693
TATACTG7450.013.6577185
TAGACTA2601.8189894E-1213.5192315
ATAGTCG1254.8500733E-513.3230
TAGACAG6400.013.2968755
Produced by FastQC (version 0.11.5)