FastQCFastQC Report
Fri 29 Apr 2016
ERR1042616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4583711
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.020.9054371
TAACGCC4800.015.4166674
GTACGAC1802.0190782E-1015.4166673
CGCGTAT855.3684966E-415.23529314
GTATTAG11200.015.0312511
ATACCGC4600.014.47826127
GTATCAA30100.014.4435222
TCGTTTA4000.014.33750130
GTATAGG7400.014.01
TCTATAC5700.013.9561413
ATAACGC5250.013.7428573
TATACTG8400.013.6547615
TACGCTG2850.013.6315795
CGCATCG4500.013.56666713
TTAACGG2601.8189894E-1213.51923235
TTAGACT3850.013.4545474
CTAGTAC4100.013.0853663
CTAACGC1703.7384052E-713.0588233
TAACGGC2703.6379788E-1213.018517536
TGCGACG2305.7661964E-1012.86956522
Produced by FastQC (version 0.11.5)