FastQCFastQC Report
Fri 29 Apr 2016
ERR1042615.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042615.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7600266
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127190.16734940592868722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104190.13708730720740564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87050.11453546494293752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40450.023.8281841
ACGGACC8800.017.659098
GACGGAC9100.016.670337
GTATCAA58800.016.2976192
TCTAGCG4900.015.47959128
TATACTG17200.015.3808145
ATAACGC7650.014.2679743
TCTATAC12600.014.0952383
GTATTAG15450.013.6504851
TAACGCC7200.013.6180564
CTACACT20000.013.5054
AAGACGG13750.013.1854545
TCAACGC73150.013.125775
CTAGCGG5950.013.05882329
ATCAACG73100.012.98294
AGACGGA12100.012.8429756
CAACGCA75150.012.7272136
TATACAG17450.012.7220635
GTACATG115350.012.7182481
CGAACGA3650.012.67123316
Produced by FastQC (version 0.11.5)