FastQCFastQC Report
Fri 29 Apr 2016
ERR1042614.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042614.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5029315
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.018.7156161
TAGACCG1251.6608647E-716.2799995
TATACTG8550.015.7953215
CTAACGC1101.4537285E-515.1363643
TAACGCC4650.015.1182794
TCTATAC5550.015.03
GTGTAAG8200.013.9878041
ACGGACC6900.013.9420298
GTATCAA27800.013.9082742
GTATTAA6150.013.83739951
TCGTTTA4300.013.76744230
GTCCTAA4900.013.5918361
CGCGCTA4900.013.59183624
GTATTAG8100.012.7901231
ACGCGCG5250.012.68571521
TATACCG1901.0482108E-712.6578955
TTATACT8350.012.6287424
CCTATAC5900.012.5423733
ATACTGT12850.012.5252926
GTATAAG6500.012.5230771
Produced by FastQC (version 0.11.5)