FastQCFastQC Report
Fri 29 Apr 2016
ERR1042609.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042609.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9022256
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT273320.30293975254082794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT232300.2574744055145409No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT175980.19505099389775685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.025.1980551
GTATCAA106700.016.8701952
TATACTG16800.015.4166665
CGCGCTA4000.015.26250124
TCTATAC15650.015.1309913
GTATTAG20250.014.6172841
TAGACCG1904.5656634E-1014.6052645
TAACGCC6100.013.647544
CTAGTAC7650.013.3006533
CGAACTA3800.013.14473729
TCTAACG2850.012.9824562
GTACTAG7100.012.7676061
GTATAGA16250.012.7507691
TTTAGCG4650.012.73118226
ATCAACG143100.012.6306784
TAATACG6150.012.3333334
CTAACGC2704.3655746E-1112.3333333
TCAACGC146750.012.2787055
ATAACGC7100.012.2464793
TAATACT24400.012.2069664
Produced by FastQC (version 0.11.5)