FastQCFastQC Report
Fri 29 Apr 2016
ERR1042608.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042608.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10394737
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT280500.26984809716686436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT243090.23385873062493068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT186310.1792349339863048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79700.024.4887071
GTATCAA119550.016.3103312
GACGGAC11400.015.5789477
ACGGACC13050.014.8850578
CGGACCA14600.013.3047949
TATACTG20150.013.2208435
GTATACG4350.013.18390751
GTATTAG22350.013.1610741
CTAATCG6250.013.02426
AAGACGG15550.012.8488745
CGAACGA5400.012.67592616
ATCAACG155950.012.5152294
TCAACGC156600.012.4632825
TAATACT24200.012.4607454
CTACACT28850.012.44020754
GTACATG207250.012.3630881
TCTATAC16650.012.3333333
TAACGCC9200.012.2663054
CAACGCA158450.012.2593876
AACGCAG160400.012.1910857
Produced by FastQC (version 0.11.5)