FastQCFastQC Report
Fri 29 Apr 2016
ERR1042607.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042607.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7019169
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT321130.45750430001044284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT285080.40614494393852035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT211200.30089031906768454No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91460.13030032472504935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94300.025.405621
TAACGCC8200.018.0487824
CGCGTAT1705.456968E-1217.41176424
ACGGACC9250.017.48
GTATCAA141200.016.8622512
GACGGAC9900.015.3232317
TGCGACG3800.015.09210622
GTACTAG6900.015.0144931
AAGACGG11750.014.9574465
CCGCGTA1001.09462766E-414.814
TCTATAC8500.014.83
CGGACCA10950.014.6986319
ATCGCGT2252.7284841E-1113.977777522
CTAACGC3050.013.9508193
ATAACGC8500.013.9294123
TAGGTCG3900.013.75641121
CGATCGT1652.600773E-713.45454513
TCGTTTA7850.013.43312130
CGTATAC3050.013.3442613
TATACTG14700.013.3401365
Produced by FastQC (version 0.11.5)