FastQCFastQC Report
Fri 29 Apr 2016
ERR1042604.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042604.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13653001
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT245400.17974070316115848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT197930.14497179045105177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT169620.12423642245393521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79200.024.1994951
GTACTAG9600.015.9947911
GTATCAA119200.015.8460572
TATACTG26000.015.7961555
TACCGTC10750.014.283727
ACGGACC14800.013.58
TCTATAC20100.013.4378123
CGTCGTA9400.013.38297910
TAATACT34200.013.1447374
CGTATTA7050.013.12056715
GACGGAC14450.013.0588247
GTATTAG30000.012.9500011
TAGGACG7050.012.8581564
TCAACGC148900.012.7474815
TAACGCC8800.012.6136364
GTACATG252900.012.5820481
ATAACGC12500.012.583
ATCAACG150650.012.5502834
ATACCGT16050.012.4485996
CAACGCA152500.012.3980326
Produced by FastQC (version 0.11.5)