FastQCFastQC Report
Fri 29 Apr 2016
ERR1042603.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042603.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9273161
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT418510.4513132037716157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT370580.3996264057099839No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT259470.27980750037662455No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122110.13168109558326443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111650.025.3349761
GTATCAA162750.017.3235032
CGCGATA1554.0199666E-1016.70967914
GTACTAG7250.015.0551731
TATACTG20200.015.0198025
CTAGTAC9900.014.9494953
TAGGTCG4750.013.242105521
TACGACG5750.013.1913045
TAGTACT13500.013.1555554
TAGACTG13950.013.1290325
GACGGAC8200.013.0853657
ATCAACG219350.012.9293364
TCTATAC14100.012.8581563
GTATTAG16550.012.8549841
TCTAACG2602.3646862E-1112.8076932
AACGCAG221800.012.8032017
TCAACGC221850.012.766965
TATACCG4800.012.7187515
CAACGCA223800.012.6557196
TATCAAC228900.012.4303183
Produced by FastQC (version 0.11.5)