FastQCFastQC Report
Fri 29 Apr 2016
ERR1042602.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042602.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5583289
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT410620.7354446456201712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT377810.6766799999068649No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT245230.4392213979967721No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145000.2597035546610609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116150.023.4136891
CTAACGC3150.018.2063483
GTACTAG5800.017.2241381
GTATCAA163800.016.6025642
TAACGCC6150.016.5447164
CAATGCG4750.015.96842119
TCTAACG3150.015.8571422
TGCGACG4800.015.80208422
TCTAGCG2600.015.65384628
ATACCGA4100.014.89024456
TAGGTCG5350.014.8691621
TATACCG3900.014.7051295
CTAGTAC7600.014.6052643
GTCGTCA5500.014.46363524
TACTAGG8350.014.4011972
TAGTACT9350.014.2459894
CTAGGCA10300.014.1893214
TACCGAC3400.014.1470597
TAGACAG8750.013.9542865
TATACTG13600.013.8755
Produced by FastQC (version 0.11.5)