FastQCFastQC Report
Fri 29 Apr 2016
ERR1042601.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042601.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12090891
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT495470.4097878311863038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT432200.35745918146148203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT312850.25874850745077427No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159100.13158666305072142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142000.025.0661981
GTATCAA205800.017.1875612
TATACTG25550.015.3502935
ACGGACC16050.014.40818
GACGGAC15350.014.3420197
TCGTTTA11150.014.26905830
TAACGCC13050.014.0344834
TATACCG5500.013.790915
AAGACGG21900.013.3470325
ATAACGC13450.013.2044623
CGGACCA18600.013.029579
TCTATAC17300.012.9393063
CCTATAC12600.012.9206353
TCTAGCG8500.012.84117728
GTATTAG21200.012.7405661
CCGGTCG12950.012.71428620
ATCAACG279350.012.6754974
AACGCAG279600.012.6641647
TCAACGC282100.012.5519325
TATCAAC289750.012.4440043
Produced by FastQC (version 0.11.5)