FastQCFastQC Report
Fri 29 Apr 2016
ERR1042600.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042600.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4634479
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT320530.6916203525790062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT264700.571153737022004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT187940.4055256265051584No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88830.1916720304482985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69800.028.8366781
GTATCAA106300.018.9524922
ATACCGA3750.015.7866666
TACCGAC3100.015.51612957
GTACTAG4500.014.81
CGCGATA1252.9632502E-614.79999914
GGACCGT3000.014.1833346
ATGCGAC3550.014.07042321
TATACTG10650.014.0704215
GTATTAG7200.013.8751
CGCGAAT1356.576789E-613.70370435
GCGCTAG1907.1431714E-913.63157911
ACGTTTA3700.013.50000126
CAATGCG3700.013.50000119
GTACCGT1102.4588802E-413.4545456
CTAGTAC4700.013.38297753
ATCAACG152850.013.2652934
CGTAGAC3650.013.1780823
TATCAAC155950.013.1320943
GGTTAGA4400.013.0340911
Produced by FastQC (version 0.11.5)