FastQCFastQC Report
Fri 29 Apr 2016
ERR1042599.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042599.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8917677
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT221460.24833821633145045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT192610.21598674183870978No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130560.14640584089331785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67650.025.9793071
GTATCAA94650.018.3534072
GACGGAC10600.014.4858497
TATACTG18150.014.1680445
TCGTTTA7300.013.93835730
GTATTAG14850.013.8282821
ACGGACC11550.013.7748928
TCAACGC129600.013.4467595
ATCAACG131200.013.3532774
ATAACGC9750.013.2820513
TAACGCC8400.013.2142854
CAACGCA132800.013.1506026
TACCGTC9850.013.1472087
TATCAAC136050.012.9044473
AACGCAG137200.012.7693157
ATTGACG11350.012.71365732
TAGACAG18450.012.3333335
TTATACT19050.012.0419944
GTGTTAG12200.011.9795081
CTATACT13850.011.8880864
Produced by FastQC (version 0.11.5)