FastQCFastQC Report
Fri 29 Apr 2016
ERR1042598.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042598.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12227583
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT281080.22987372075086304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT231060.18896620861211902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT191680.15676033440132853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84350.026.1653821
GTATCAA128350.017.0370082
TCTAGCG8300.016.93975828
GACGGAC17700.016.0960457
ACGGACC19200.015.6093748
CTAGCGG9650.015.14507829
TCTATAC16750.015.1313453
TATACTG24150.014.9378885
TCGTTTA10300.013.83009630
CGCAATA11600.013.55603436
AAGACGG24800.013.5020165
TATACCG5100.013.4215695
TAGTACT14200.013.2887334
CTAGTAC10800.013.1898143
ATCAACG168150.013.0264644
CGGACCA22750.013.0109899
TCAACGC170050.012.8700395
CGCGCTA15550.012.84887524
GTATTAG25000.012.6541
CGAACGA8300.012.48192816
Produced by FastQC (version 0.11.5)