FastQCFastQC Report
Fri 29 Apr 2016
ERR1042597.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042597.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5343194
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT156120.2921847868522086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144750.2709053798158929No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71700.1341894005720174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58200.019.1357381
ACGGACC8700.017.4367838
GACGGAC9000.016.4444457
CGGACCA10550.015.2559249
GTATCAA73650.014.9708082
TATACTG10600.014.8349065
CGTCGTA4150.014.71084310
TACGACG4850.014.4948455
TAACGCC6150.014.4390254
TACCGTC5000.014.4299997
AAGACGG12400.014.322585
TAAGACT7800.013.9935884
AGACGGA11650.013.9742496
TCGTTTA6250.013.91230
ATAACGC7900.013.8164563
GTATTAG12300.013.6869931
CTTACAC9600.013.4895843
TACACTG19100.013.463355
TCTATAC7300.013.4315073
GTAAGAC6750.013.429633
Produced by FastQC (version 0.11.5)