FastQCFastQC Report
Fri 29 Apr 2016
ERR1042596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10982243
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT371660.3384190278798238No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT361030.3287397665486003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237120.21591217750326597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111760.10176427529421812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134550.018.6443711
TATACTG18900.014.0952375
TCTATAC14550.013.9862543
GTATCAA183300.013.6151112
GTACTAG9000.013.5666671
CTAGCGG7800.013.51923129
GTATTAG21600.013.4467591
TCTAGCG7750.013.36774328
TAACGCC10750.013.25116354
CTAATAC24800.013.0544353
TGCGACG5700.012.98245622
GTAAACG9750.012.52307727
GTATAAG18350.012.4005451
TAGACTG15100.012.2516575
CTAGTAC10150.012.21182253
CTACACT31200.012.1554494
GACGGAC12900.012.0465137
CGCGCTA7850.012.01910924
GTACATG235400.011.9377661
AACGCAG210250.011.9315117
Produced by FastQC (version 0.11.5)