FastQCFastQC Report
Fri 29 Apr 2016
ERR1042595.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042595.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8134651
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT192440.23656823138448105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT188840.2321427188455903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120190.14775065334702128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73000.018.9308221
TATACTG13950.015.25089555
TAACGCC8150.014.7546014
ACGGACC11250.014.6355558
TCGTTTA7650.014.50980430
CGAACGA3450.014.47826116
TAATACT19050.014.0813654
GACGGAC11200.014.0401797
GTATCAA100000.013.8752
GTATTAG17850.013.2661071
GTACTAG5900.013.1694921
CGTATAC2703.6379788E-1213.0185183
CTTACAC13800.012.7355083
CTAGTAC5700.012.6578943
CTAATAC18600.012.6317213
TACACCG7250.012.50344855
TCTATAC10650.012.3333333
CTACACT21250.012.2752944
ATAACGC10300.012.2135933
CGTCTAT4400.012.1931821
Produced by FastQC (version 0.11.5)