FastQCFastQC Report
Fri 29 Apr 2016
ERR1042594.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042594.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10209902
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT176630.17299872222084012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT174920.17132387754554354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118820.1163772188998484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79250.017.8580441
TATACTG17050.015.9501475
GTAAACG9000.015.62222227
ACGGACC13650.015.450558
TAACGCC11600.014.8318974
TAATACT18200.014.33241754
GTATTAG21400.014.2640181
ATAACGC11700.014.230773
GTATACG5200.014.2307691
TATACCG4450.014.134835
GACGGAC14300.014.1013987
TCTATAC13350.013.8576783
TACGACG9150.013.7486335
GTATTAA14200.013.6795781
TACACCG8600.013.5523255
TACCGTC9850.013.3350267
TCGTTTA9900.013.26767730
ATACCGT12350.013.1821876
TCTAGCG8450.013.13609428
ACGACGG9450.013.1164036
Produced by FastQC (version 0.11.5)