FastQCFastQC Report
Fri 29 Apr 2016
ERR1042593.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042593.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9549241
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT134340.1406813379199457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT125190.131099424551124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73300.016.3294681
TCGCTAA2450.014.3469414
TAACGCC9500.014.215794
GACGGAC13200.013.5946967
TATACTG16500.013.4545465
TACGACG8300.013.1506025
ACGGACC13400.013.1156718
CTTACAC17250.012.8695653
GCGTTAG2451.1459633E-1012.8367361
ACGACGG8100.012.7901236
TCTAGCG7500.012.58000128
TCGTTTA9450.012.529130
TTAACGG3550.012.50704335
CGCGCTA9050.012.46961324
GTATTAG20050.012.4563591
GTAAACG8200.012.40853627
ATAACGC11100.012.3333343
TCTATAC11150.012.2780273
CTAGTAC8000.012.256253
TCTAGAC10500.012.1571443
Produced by FastQC (version 0.11.5)