FastQCFastQC Report
Fri 29 Apr 2016
ERR1042589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5474477
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT191550.349896437595774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT188080.34355793256597844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT109720.20042097172022094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72200.020.3448751
GCGCGAA656.908196E-519.92307711
GTAAACG4300.018.527
ACGGACC7400.018.258
GACGGAC7950.017.6855357
CGGACCA8500.016.323539
GTACTAG3650.016.219181
TTAACGG3000.015.41666735
CGCAAGA9650.015.3367882
AAGACGG10750.014.7999995
TAGACAG10300.014.7281545
TAACGGC3150.014.68253936
TACCGTC5950.014.6134457
GTATCAA100000.014.5782
GTATTAG14300.014.1013981
TATACTG10800.014.0462955
CGTCGTA5050.013.92079310
TCTATAC8000.013.8750013
TTAGACT6000.013.8754
ATACCGT8600.013.7674426
Produced by FastQC (version 0.11.5)