FastQCFastQC Report
Fri 29 Apr 2016
ERR1042588.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042588.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3721688
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT205910.5532704514725576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT188220.5057382564040833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76840.2064654533104333No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54870.14743310024913425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56150.024.5788081
GTATCAA70450.019.5372622
CGCGATA1409.458745E-1118.514
ACGGACC4900.017.7448988
GACGGAC5750.015.7652187
CGGACCA5700.015.5789479
AGTACCG2450.015.102045
TCTATAC4050.015.0740743
TATACTG6800.014.9632365
TATACCG2006.184564E-1114.85
TACACTG11500.014.4782615
ACGTGCG2059.276846E-1114.4390249
TAGACTG5000.013.6900015
AACGCAG103100.013.6013587
GTAGCAC4100.013.5365853
ATCAACG104750.013.4047734
CGCAAGA7650.013.3006532
TCAACGC106050.013.2578975
GTGTAAG7700.013.2142851
CAACGCA107100.013.1279176
Produced by FastQC (version 0.11.5)