FastQCFastQC Report
Fri 29 Apr 2016
ERR1042587.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042587.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886084
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT130730.6931292561731078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116520.6177879670258589No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42490.2252815887309367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41950.2224185137035254No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT25870.1371625017761669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31850.025.3249591
GTATCAA37900.021.2823222
GTAATAC2950.020.0677973
ACGGACC4800.020.0416668
CGAACGA1650.019.06060616
ACGAACG1650.019.06060615
GACGGAC4900.018.8775527
CGGACCA4750.018.6947369
CGCGATA609.239811E-418.514
ACGTGCG1002.8773502E-718.59
GTAAGAC2250.017.2666663
ATAACGA2000.016.65000212
GCGAAAG5250.016.56190518
CGTACGC904.448388E-516.4444453
TAAGACT2700.016.4444434
TAATACT5900.016.3050844
TATATCG1251.6592458E-716.2799995
CTAATAC6950.016.237413
CGAAAGC5350.015.90654219
CCGATAA2100.015.8571429
Produced by FastQC (version 0.11.5)