FastQCFastQC Report
Fri 29 Apr 2016
ERR1042586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8685986
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT191560.22053915352845377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT175830.20242952268170822No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118750.13671447317552665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70000.020.4821431
TATACTG16250.014.6861535
TAACGCC8300.014.4879524
GTATCAA97650.014.3604722
ACGGACC9700.014.3041248
TCTATAC12150.014.1604943
CTAGTAC6050.013.7603313
ATAACGC10650.013.2018773
GACGGAC10250.012.9951227
TACCGTC7550.012.7417227
TCGTTTA7750.012.65161230
CGTATTA4600.012.46739215
TACACTG29550.012.4585455
GTGTAAG16300.012.3711651
TAATACT18850.012.3660484
CTACACT24450.012.1820044
CGCGCTA6500.011.95384724
ATACCGC8850.011.91525427
TACGACG7300.011.9109595
GTACTAG6700.011.8731351
Produced by FastQC (version 0.11.5)