FastQCFastQC Report
Fri 29 Apr 2016
ERR1042585.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042585.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744553
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19370.1110313071600576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG352.3890123E-531.7142871
GGTATCA11800.023.830511
TATACTG2300.023.3260885
GCGCGTT450.00382659120.55555511
ACGGACC3750.020.2266658
CGCAAGA4100.019.8536572
GACGGAC3850.019.7012987
GTATCAA14050.019.22422
ATCGATA609.2394307E-418.514
CGGACCA4150.017.8313249
GTATACT2300.017.6956524
CGAACTA650.001580377517.07692324
GCGCAAG5000.016.651
GCGAAAG4450.016.62921318
ACCGGTA904.448081E-516.44444521
AGCGAAA4550.016.26373517
GTCGTAC700.00259321515.8571431
CGAAAGC4800.015.80208319
TATCTAG1302.591005E-715.6538461
AGACGGA5250.015.5047626
Produced by FastQC (version 0.11.5)