FastQCFastQC Report
Fri 29 Apr 2016
ERR1042584.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042584.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21561301
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT510170.23661373680558515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT416120.19299391998655369No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT388180.18003551826487652No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263090.12201953861689516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157700.023.3214971
GTATCAA244850.015.0130692
ACGAACG14150.013.204946515
CGAACGA13900.013.17625916
CTTATAC81750.013.17064237
CTAGCGG15700.012.6082829
TATACTG59150.012.5418435
GACGGAC26800.012.4944037
ACGGACC27700.012.489178
TACGACG17900.012.4022355
TCTATAC32700.012.2201833
TCTAGCG15950.012.17868428
ATACCGT25050.011.8163686
ATCAACG313350.011.6661884
TCAACGC315500.011.6042795
GCGTTAT21150.011.54611
TATACCG9300.011.5376345
TATCAAC325000.011.5098473
GTATTAG41350.011.4534451
TCTTATA129450.011.40440337
Produced by FastQC (version 0.11.5)