FastQCFastQC Report
Fri 29 Apr 2016
ERR1042583.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042583.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054295
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50090.4751042165617782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24330.23077032519361282No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG19790.18770837384223582No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT17850.1693074518991364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16460.15612328617701876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.12776310235749958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG250.0054961429.64
TATACCG250.0054961429.65
GGTATCA18650.024.6997321
TCGTTCG3450.021.44927437
CGTATAC450.00382560220.5555553
CCGTATG450.00382560220.5555552
GTATCAA22550.020.3458982
TACAGCG555.1427056E-420.1818187
ACTATAC1202.382876E-1020.0416663
ACGGACC3700.020.08
CGGTAAC656.9014386E-519.9230772
TCGCGTA4900.019.6326529
CGCGTAA5000.019.60999910
GTGTTAT851.2451073E-619.5882361
CGGACCA4000.018.9625029
GTCGCGT5100.018.8627458
TACTCCG801.6164631E-518.55
GACGGAC4000.018.03757
GCGTAAC5250.017.9714311
AAGACGG5050.017.9504955
Produced by FastQC (version 0.11.5)